RBRC02975 三菱化学生命科学研究所・中村健司先生、東京都臨床医学総合研究所・水島　昇先生（2003）。CCE/EK.CCE ES細胞を用いて作出。C57BL/6Jへ戻し交配された。 Atg5遺伝子のfloxedマウス。第3エクソンの両端にloxPサイトが挿入され、3エクソンの下流にneoカセットを持つ。Atg5の発現は低下しているが、オートファジーには影響しない。creによりホモ欠損体になるとオートファジーが不能となる。ノックアウマウス (nullアレル) もあり (RBRC02231 B6;129-Atg5<tm1Nmz>) 。 Phage P1 LoxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neo, mouse Atg5 genome DNA Atg5 floxed mice. Exon 3 of Atg5 gene was flanked by loxP sites containing neomycin resistant cassette. Autophagy is an intracellular degradation process by an autophagosome, which contains a portion of cytoplasm and subsequently degrades upon fusion with a lysosome. Autophagy is considered to be important for the cellular response to starvation and the normal turnover of cytoplasmic components as well. Atg5, autophagy-related 5 is essential for autophagosome formation. Conditional B6.129S-Atg5<tm1Myok> (RBRC02975) mice can be crossed with various tissue-specific Cre transgenic mice to study the role of autophagy in adult tissues. Null knockout mice are also available: B6;129-Atg5<tm1Nmz> (RBRC02231). true B（1〜3か月） <a href='https://brc.riken.jp/mus/pcr02975'>Genotyping protocol -PCR-</a> 水島　昇 Cell Biology Research B (1-3 months) Cre/loxP system Noboru MIZUSHIMA Atg5遺伝子のfloxedマウス。第3エクソンの両端にloxPサイトが挿入され、3エクソンの下流にneoカセットを持つ。Atg5の発現は低下しているが、オートファジーには影響しない。creによりホモ欠損体になるとオートファジーが不能となる。ノックアウマウス (nullアレル) もあり (RBRC02231 B6;129-Atg5<tm1Nmz>) 。, <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/jul_2009_mm" target="_blank">Mouse of the Month Jul 2009</A><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/02975_GB.pdf">Genetic Background</a><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/02975_BLC.pdf">Breeding characters</a> Atg5flox Atg5flox Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 441, 885-889 (2006).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization.　Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution.　The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. CCE/EK.CCE [129S/SvEv-Gpi1<c>] Developed by Kenji Nakamura, Mitsubishi Kagaku Institute of Life Sciences and Noboru Mizushima, Tokyo Metropolitan Institute of Medical Science in 2003. The construct was electoroporated into CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c>. The mutant mice were backcrossed to C57BL/6J. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://molbiolut.jp/en/" target="_blank">Lab HP</A> B6.129S-Atg5<tm1Myok> B6.129S-Atg5<tm1Myok> 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Nature, 441, 885-889 (2006).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>非営利団体による学術研究に限る。当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に 寄託者との間で、別途使用許諾に係る契約の締結が必要である。利用者が本件リソースを使用してえられた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。